Browsing by Author "BEN GHOULA, Chaima"

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    Etude phytochimique et activités biologiques de l’extrait méthanolique de Teucrium chamaedrys L.
    (Faculté des sciences de la nature et de la vie et des sciences de la terre, 2024) BEN GHOULA, Chaima; BEN KHELIFA, Karima
    Teucrium chamaedrys L. is a plant widely used in traditional medicine known as "Balut al arth". The present work aims to study the phytochemical composition and evaluate the antioxidant, antibacterial and anti- inflammatory activities of the methanolic extract of this plant. Extraction was carried out by Soxhlet and the yield was 10.75±1.76%. The colorimetric method revealed that the extract was rich in total polyphenols (229.27±5.85μg EAG/mg Ex) and flavonoids (40.85±0.43μg EQ/mg Ex). The antioxidant activity results showed that the methanolic extract possessed a powerful antioxidant capacity with a CAT of 310.07±6.06μg EAA/mg Ex. In addition, the extract exhibited a high antiradical capacity towards DPPH and ABTS radicals (IC50=28.27±0.87μg/mL and 317.20±2.45μg/mL, respectively). The extract exerted significant reducing power (A0.5=116.19±5.63) and remarkably inhibition of β-carotene bleaching (AA%=77.08±2.63%). Antibacterial activity results showed good activity against B. cereus (MIC=0.39mg/mL). The results of the in vitro anti-inflammatory activity showed that the methanolic extract exhibited significant inhibitory power (IC50= 437.80±21.11μg/mL). On the other hand, the evaluation of the anti-inflammatory activity in vivo significantly reduced oedema formation, with the highest percentage observed at the dose of 400mg/kg (75.84±5.61%). These results justify the traditional use of this plant, which merits further study.