Étude comparative chimiotaxonomique basée sur les coefficients de Dice et d’Ochiai et moléculaire du genre Microbispora

Abstract

The study was structured around two main axes: on the one hand, a chemotaxonomic analysis based on the similarity coefficients of Dice and Ochiai; on the other hand, a molecular approach relying on the 16S rRNA gene sequence. It conducted on thirteen species from the phylum Actinomycetota, all belonging to the genus Microbispora, namely: M. rosea, M. catharanthi, M. saimensis, M. hainanensis, M. bryophytorum, M. triticiradicis, M. camponoti, M. soli, M. sitophila, M. clausenae, M. oryzea, M. maris, and M. corallina. The objective of the study was to conduct a comparative analysis among Microbispora species, and to identify the species most closely related to Microbispora maris (considered the reference), through a comparison of chemotaxonomic and molecular analysis results. For this purpose, the Ochiai and Dice coefficients were applied to data derived from the chemical composition of the cell walls, while bioinformatic algorithms were used to align nucleotide sequences and estimate genetic distances. The results revealed a partial discrepancy between the two approaches. The chemotaxonomic analysis indicated that M. catharanthi was the closest species to M. maris, whereas the molecular analysis identified M. rosea as the most closely related species. This study confirms that the two approaches are complementary for taxonomic classification purposes.