Activités antimicrobiennes de certaines plantes utilisées dans le traitement des infections urinaires dans la région de Ghardaïa

Abstract

Plants are an inexhaustible source of bioactive substances that can replace molecules lose their business because of the intense and uncontrolled use. Among these antibiotics molecules which become increasingly inactive microorganisms, because they develop mechanisms which enable them to resist the action of these molecules. In this study, we try to evaluate the antimicrobial activity of extracts from two wild species of the northern Sahara traditionally used in treating urinary tract infections that are PeriplocaangustifoliaLabil andCymbopogonschoenanthus L. The extraction of active ingredients was carried out by two traditional methods of preparation: infusion and decoction with two solvent water and ethanol. The determination of levels of polyphenols and flavonoids has been performed by the Folin-Ciocalteau method and Kim et al. (2003) respectively. The evaluation of the antimicrobial activity is made by the disk diffusion method on agar of 4 bacterial strains that are Escherichia coli (ATCC 10536), Klebsillapneumoniae (CIP 82.91), Pseudomonas aerouginosa (CIP A22), Staphylococcus aureus ( CIP 7625), and which is a yeast Candida albicans (API 200). The results showed that the extract by boiling with ethanol Cymbopogonschoenanthus L. has the highest levels of polyphenols and flavonoids which are respectively 31.08 ± 1.57 mg EAG / g PS and 10.80 ER ± 0.40 mg / g PS. The infusion with ethanol PeriplocaangustifoliaLabil.has the highest content PPt (35.69 ± 2.41 mg EAG / g PS), while the aqueous infusion has the highest flavonoid (6.88 ± 0.50 mg RE / g PS content ). Different extracts have an average antimicrobial activity on the tested strains.