Evaluation de l’activité antifongique des extraits de feuilles d’olivier contre les agents de mycoses

Abstract

In Algeria, olive tree (Olea europea. L) is considered one of the most widely used medicinal plants in traditional treatments against various diseases, particularly fungal infections. The objective of this study is to evaluate the antifungal properties of extracts from two varieties of olive leaves (Chemlal and Sigoise) harvested in two different regions of the Ghardaïa province. Maceration with methanol-water followed by rotary evaporation revealed a considerable yield for the extract of the variety Chemlal reaching 27.77%. The quantitative analysis of total polyphenols and flavonoids in the extracts revealed that the variety Sigoise has higher polyphenol content than the Chemlal variety, reaching up to 117.64 μg EAG/mg EX, while the flavonoid concentrations are nearly equal. Antifungal activity was determined against three Candida strains (Candida albicans 307, C. albicans 384, and C. albicans ATCC 10231) using the well diffusion method. According to the results, the extracts from both varieties inhibit the growth of fungal strains. Determine of the minimal inhibitory concentration (MIC) and Minimum fungicidal concentration (MFC) shows that C. albicans 384 strain is the most resistant. The composition of the extracts in bioactive compounds (polyphenols and flavonoids) could explain their significant activity against fungal infections.